Introduction Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics. • Reduced background signal. I: Input, FT: Flow-Through, B: Bound. 同 B. Only for research applications, not for diagnostic or therapeutic use 1. GFP-Trap®_MA manual - 1 - Version 2017-10-04 GFP-Trap®_MA for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. The binding rate of GFP-Trap however is very high. Immunoprecipitation is a common first step for experiments such as selectively enriching for phosphoproteins or peptides for post-translation modification studies or concentrating a biomarker prior to quantitation. I: Input, FT: Flow-Through, B: Bound.-/ -Cat No. GFP-Trap For immunoprecipitation of GFP-fusion proteins Referenced in about 1,600 publications. I am encountering a weird problem in my immunoprecipitation experiments with GFP magnetic beads. GFP-Trap® Dynabeads（磁気ビーズ標識GFP抗体） 商品名: GFP-Trap® Dynabeads（GFPトラップ®ダイナビーズ） （Nano-Trap® alpaca anti-GFP, recombinant VHH, coupled to Dynabeads） Enlarge Image × Immunoprecipitation of GFP with GFP-Trap Agarose beads. GFP-Trap. .. Co-transfection of vMIA-myc and GFP-N1 was used as negative control. Based on the properties of the different matrices (see table), we recommend: GFP-Trap Agarose for very low background and high binding capacity IP; GFP-Trap Magnetic Agarose for magnetic separation and high binding capacity IP; GFP-Trap Dynabeads for IP of very large proteins/complexes; GFP-Trap Multiwell … Introduction localization and dynamics. GFP-Trap for Immunoprecipitation Anti-GFP antibody to perform immunoprecipitation of GFP fusion proteins and their interaction factors. The immunoprecipitation is performed with anti-DYKDDDDK® antibody coupled to agarose beads, which are generated by covalently linking agarose to a highly specific mouse monoclonal antibody raised against DYKDDDDK. Anti-DYKDDDDK Kit for Immunoprecipitation allows a rapid and efficient immunoprecipitation and elution of an active DYKDDDDK -tagged recombinant protein in less than 2 hours. GFP-Trap®_MA kit manual - 1 - Version 2017-10-04 GFP-Trap®_MA for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. GFP-Trap®_A manual - 1 - Version 2017-10-04 GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. Immunoprecipitation of GFP with GFP-Trap Agarose beads. SKU/Size. GFP-Trap’s affinity has been measured as 1 pM (Figure 4). Introduction Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics. Immunoprecipitation For the c-myc-Ago2 immunoprecipitation ex periments, rat Schwann cells wer e transfected with the c-myc-Ago2 plasmid and either the negative (Neg.) 免疫沈降（IP; ImmunoPrecipitation）は抗原と抗体の親和性を利用して、溶液中から抗原を特異的に分離させる方法です。免疫沈降で分離した抗原はSDS-PAGEやウエスタンブロッティングなどの方法で解析します。 原理. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™. In... Matrix. The combination of low disscociation and high binding rates results to a very low K D respectively high affinity. 适用性. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. The same 1-step purification procedure was then applied to GFP fused to the protein PCNA in a co-immunoprecipitation experiment. For biochemical analysis including mass spectrometry and enzyme activity measurements, TurboGFP-fusion proteins and their interacting factors can be isolated fast and efficiently by immunoprecipitation using the TurboGFP-Trap. 同A. Casp2pro BiFC cells were again treated with caspase‐2‐activating stressors, lysed, and incubated with GFP‐Trap. GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins For the immunoprecipitation of GFP-fusion-proteins from cellular extracts. Load more search results . Quantity. The GFP-Trap has a very low dissociation rate; only very small amounts of GFP dissociates even during lengthy washing. GFP-Trap is an anti-GFP antibody fragment conjugated to magnetic beads. For a specific protein or complex, the optimal experimental conditions/ GFP-Trap have to be tested. 可选用特异性抗体 或 GFP 单克隆或多克隆抗体 +Protein A/G 琼脂糖微珠. GFP 抗体-琼脂糖珠子， RFP-Trap ， GST-Trap ， Dnmt1-Trap. Low background*. The GFP-Trap® is a monovalent matrix (agarose or magnetic beads) with GFP-binding proteins coupled to it. Introduction localization and dynamics. The GFP Tag Immunomagnetic Beads, conjugated with GFP Antibody（13105-R208）, are used for Immuneprecipitation / IP of GFP-tagged proteins expressed in vitro expression systems. As expected, the bound fractions containing GFP are extraordinarily pure. GFP‐Trap immunoprecipitation of caspase‐2 BiFC dimers identifies active caspase‐2‐interacting proteins. More than 1,800 publications peer reviewed articles have already been published using ChromoTek's GFP-Trap. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. Affinity purification ChIP and related techniques ELISA GFP High content analysis Immunofluorescence Immunoprecipitation Ligand capture and Affinity Determination Methods Live Cell Imaging Mass spectrometry On-bead enzyme assays Spot Capture and Detection System Super … scrambled miR or miR-29a (Karginov et al. Its advantages are as follows: • Extremely efficient reduction of GFP fusion proteins. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (magnetic agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. For immunoprecipitation of Pex19-YFP the GFP-Trap_M kit (Chromotek, Planegg-Martinsried, Germany), consisting of a high quality GFP-binding protein coupled to a monovalent matrix of magnetic agarose beads, was used. GFP-Traps® are ideal for ... RNA Immunoprecipitation (RIP) GFP-Trap® - Single band purification of GFP. 产物. Size GFP-tagged protein*. Immunoprecipitation is a highly selective technique which can be used to enrich or concentrate low abundance species from a complex matrix. 1-888-478-4522 proteintech@ptglab.com . 4th … For IP, the Immunomagnetic Beads are added to a sample containing GFP-tagged proteins to form an Immunomagnetic Beads-protein complex. Les GFP-Traps® sont idéales pour l'isolation efficace en une seule étape des protéines de fusion fluorescentes et des partenaires qui interagissent avec elles (voir le schéma global ci-dessous). Dynabeads M-270 Bead form. 同A. Immunoprecipitations (IP) of GFP from protein extracts of human cells. 有 GFP ， RFP ， GST ， Dnmt1-Trap 等 4 个品种的标签蛋白 Trap 可选. Produktmerkmale Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. GFP-Trap®_A manual - 1 - Version 2017-10-04 GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins from mammalian cell extract Only for research applications, not for diagnostic or therapeutic use. 通用方法，适合面宽. Applications. Immunoprecipitation with GFP-Trap. Freight/Packing:-Country/Region. Comparison of GFP-Traps® with conventional mono- and polyclonal antibodies. I: Input, FT: Flow-Through, B: Bound. gta. Proteintech North America (HQ) Proteintech Group, Inc 5400 Pearl Street, Suite 300 Rosemont, IL 60018, USA. Le GFP-Trap® est une matrice monovalente (agarose ou billes magnétiques) couplée à des protéines qui se lient aux GFP. 同 B. 2007). gfp 2,489 citations have been found for this product All applications Affinity chromatography (Affin Chroma) Binding experiments (Binding) Chromatin immunoprecipitation (ChIP) Functional assays (Functional) Immunocytochemistry-immunoflourescence (ICC-IF) Immunopurification (Immu-puri) Immunoprecipitation (IP) Pull-Down Assay (Pull-Down Assay) RNA Immunoprecipitation (RNA-IP) … The complex is removed from the solution manually against … No binding to proteins derived from DsRed, all RFPs and TurboGFP can be detected. Introduction localization and dynamics. • More than 1,500 publications. All in less than 4 hours. Magnetic GFP-Trap-M Kit, for GFP Fusion Protein Immunoprecipitation (20 tests) CHT gtmk-20 B 1 kit / 77,000 GFP結合ビーズ別売品 品 名 メーカー 商品コード 包装 /価格（￥） GFP-Trap-A CHT gta-20 20 tests / 61,000 Magnetic GFP-Trap-M CHT gtm-20 20 tests / 61,000 抽出 精製 使 用 例 Choose from 3 GFP-Trap (50 µL, 2 reactions) sample types: • GFP-Trap Agarose (for very low background and high binding capacity IP) GFP-Trap®_A manual - 1 - Version 2009-09-28 GFP-Trap®_A for Immunoprecipitation of GFP-Fusion Proteins (gta) For the immunoprecipitation of GFP-fusion proteins from cellular extracts. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. Binding capacity. Proteintech Europe. The ability to specifically capture caspase‐2 dimers afforded an optimal system to isolate and characterize the interactome of dimerized caspase‐2. Bead separation. The ChromoTek GFP-Trap provides fast, reliable, and e ffective immunoprecipitation of GFP-fusion proteins. Turbo-GFP shares only ~21 % sequence identity with the commonly used GFP variants. Ligand. We describe the GFP-Trap immunoprecipitation as a cell-based method to analyze the OTUD6B-OTUB1 interaction in the cellular context and the AlphaScreen (amplified luminescent proximity homogeneous assay) assay as a tool to detect direct interactions and to search for PPI inhibitors. 纯度不高. Introduction localization and dynamics. Add to Basket Add to Quote INQUIRE FOR PRICING. GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (magnetic agarose beads) for biochemical analysis of GFP fusion proteins and their interacting partners. Only for research applications, not for diagnostic or therapeutic use 1. GFP-Trap® specifically binds to eGFP, wtGFP, GFP S65T, TagGFP, eYFP, YFP, Venus, Citrin, CFP. What GFP-Trap should I use for my immunoprecipitation? iST-GFP-Trap Kit for immunoprecipitation and sample preparation for mass spectrometry. GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. 10 LIST OF ABBREVIATIONS Adh ARC CCA1 ChIP c-Myc CRSP CTD DRIP EST GFP GUS HAT Hsp18.2 IgG PC2 PIC RNA pol Sep10 SMCC Soh1 SWI/SNF TAP TFIIA TFIIB alcohol dehydrogenase activator-recruited cofactor circadian clock associated 1 chromatin immunoprecipitation cellular myelocytomatosis oncogene cofactor required for Sp1 activation carboxy-terminal domain vitamin D receptor interacting … The PreOmics / ChromoTek iST-Trap Kit enables the fast and efficient immunoprecipitation of GFP-Fusion proteins and the interaction factors followed by the innovative sample preparation for mass spectrometry based proteomics.
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